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pSLIK_Zeo_3xflag_Luciferase
(Plasmid #136533)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136533 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSLIK-Zeo
  • Backbone manufacturer
    Addgene #25736
  • Backbone size w/o insert (bp) 12944
  • Vector type
    Mammalian Expression, Lentiviral ; destinatioin vector for gateway cloning
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Bleocin (Zeocin), 100 & 25 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    **Grow plasmid in amp+ 25 ug/ml zeo in low-salt LB at 30degC.**
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Luciferase
  • Species
    Firefly
  • Tag / Fusion Protein
    • 3x FLAG (N terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSLIK_Zeo_3xflag_Luciferase was a gift from Kevin Janes (Addgene plasmid # 136533 ; http://n2t.net/addgene:136533 ; RRID:Addgene_136533)

  • For your References section:

    Sporadic activation of an oxidative stress-dependent NRF2-p53 signaling network in breast epithelial spheroids and premalignancies. Pereira EJ, Burns JS, Lee CY, Marohl T, Calderon D, Wang L, Atkins KA, Wang CC, Janes KA. Sci Signal. 2020 Apr 14;13(627). pii: 13/627/eaba4200. doi: 10.1126/scisignal.aba4200. 10.1126/scisignal.aba4200 PubMed 32291314
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