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pGEM_ZmaBOP2binsitu
(Plasmid #136532)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136532 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM T-easy
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 4500
  • Vector type
    Cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zoysia matrella BLADE-ON-PETIOLE homolog2b
  • Alt name
    ZmaBOP2b
  • Species
    Zoysia matrella wakaba
  • Insert Size (bp)
    1500

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer M13 Forward
  • 3′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM_ZmaBOP2binsitu was a gift from Junko Kyozuka (Addgene plasmid # 136532 ; http://n2t.net/addgene:136532 ; RRID:Addgene_136532)

  • For your References section:

    Suppression of Leaf Blade Development by BLADE-ON-PETIOLE Orthologs Is a Common Strategy for Underground Rhizome Growth. Toriba T, Tokunaga H, Nagasawa K, Nie F, Yoshida A, Kyozuka J. Curr Biol. 2020 Feb 3;30(3):509-516.e3. doi: 10.1016/j.cub.2019.11.055. Epub 2020 Jan 16. 10.1016/j.cub.2019.11.055 PubMed 31956025
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