Lyn-tdnano-HA
(Plasmid
#136512)
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PurposeMammalian expression of a tandem dimer of WT SspB (nano), targeted to membrane by Lyn myristoylation sequence, with triple-HA epitope tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 136512 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP N1
- Backbone size w/o insert (bp) 900
- Total vector size (bp) 4800
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLyn nano nano 3HA
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Alt nameSspB
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Insert Size (bp)900
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bySspB WT (nano) received from lab of Brian Kuhlman via Addgene (60415)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lyn-tdnano-HA was a gift from Bianxiao Cui (Addgene plasmid # 136512 ; http://n2t.net/addgene:136512 ; RRID:Addgene_136512) -
For your References section:
Construction of Light-Activated Neurotrophin Receptors Using the Improved Light-Induced Dimerizer (iLID). Hope JM, Liu A, Calvin GJ, Cui B. J Mol Biol. 2020 Jun 12;432(13):3739-3748. doi: 10.1016/j.jmb.2020.04.018. Epub 2020 Apr 23. 10.1016/j.jmb.2020.04.018 PubMed 32335036
Map uploaded by the depositor.
