iLID-mRuby3-iTrkA
(Plasmid
#136510)
-
PurposeMammalian expression of iLID fused to mRuby3 and intracellular signaling domain of TrkA (aa 450-799)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 136510 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepEGFP N1
- Backbone size w/o insert (bp) 3900
- Total vector size (bp) 6250
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameiLID mRuby3 iTrkA
-
Alt nameTrkA
-
SpeciesR. norvegicus (rat); Avena sativa
-
Insert Size (bp)2250
-
Entrez GeneNtrk1 (a.k.a. Trk)
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made byiLID received from lab of Brian Kuhlman via Addgene (60411)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
iLID-mRuby3-iTrkA was a gift from Bianxiao Cui (Addgene plasmid # 136510 ; http://n2t.net/addgene:136510 ; RRID:Addgene_136510) -
For your References section:
Construction of Light-Activated Neurotrophin Receptors Using the Improved Light-Induced Dimerizer (iLID). Hope JM, Liu A, Calvin GJ, Cui B. J Mol Biol. 2020 Jun 12;432(13):3739-3748. doi: 10.1016/j.jmb.2020.04.018. Epub 2020 Apr 23. 10.1016/j.jmb.2020.04.018 PubMed 32335036