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Addgene

pGEX2T E7 L67E
(Plasmid #13651)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13651 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX2T
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HPV 16 E7 L67E
  • Alt name
    HPV 16 E7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    300
  • Mutation
    aa 67 from L to E
  • GenBank ID
    AY089955
  • Entrez Gene
    E7 (a.k.a. HpV16gp2)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer Amersham pGEX 5'
  • 3′ sequencing primer Amersham pGEX 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX2T E7 L67E was a gift from Karl Munger (Addgene plasmid # 13651 ; http://n2t.net/addgene:13651 ; RRID:Addgene_13651)

  • For your References section:

    The human papillomavirus E7 oncoprotein and the cellular transcription factor E2F bind to separate sites on the retinoblastoma tumor suppressor protein. Wu EW, Clemens KE, Heck DV, Munger K. J Virol. 1993 Apr . 67(4):2402-7. PubMed 8445736
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