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Addgene

pPS2167
(Plasmid #1365)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1365 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pPS1629
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RPL11B
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    RPL11B
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CEN vector with ORF of GFP followed by the 3'UTR of NUF2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS2167 was a gift from Pamela Silver (Addgene plasmid # 1365 ; http://n2t.net/addgene:1365 ; RRID:Addgene_1365)
  • For your References section:

    Factors affecting nuclear export of the 60S ribosomal subunit in vivo. Stage-Zimmermann T, Schmidt U, Silver PA. Mol Biol Cell 2000 Nov;11(11):3777-89. 10.1091/mbc.11.11.3777 PubMed 11071906