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PurposeLentiviral expression of AsCas12a gRNA
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 136474 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLKO
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Vector typeLentiviral, Synthetic Biology
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePuromycin resistance
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gRNA/shRNA sequenceBsmBI Cloning cassette
- Promoter EF1a
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRDA_052 was a gift from John Doench (Addgene plasmid # 136474 ; http://n2t.net/addgene:136474 ; RRID:Addgene_136474) -
For your References section:
Optimization of AsCas12a for combinatorial genetic screens in human cells. DeWeirdt PC, Sanson KR, Sangree AK, Hegde M, Hanna RE, Feeley MN, Griffith AL, Teng T, Borys SM, Strand C, Joung JK, Kleinstiver BP, Pan X, Huang A, Doench JG. Nat Biotechnol. 2020 Jul 13. pii: 10.1038/s41587-020-0600-6. doi: 10.1038/s41587-020-0600-6. 10.1038/s41587-020-0600-6 PubMed 32661438
