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Addgene

pKD077
(Plasmid #136473)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136473 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Backbone size w/o insert (bp) 5382
  • Total vector size (bp) 6639
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SSB-mTur2
  • Species
    E. coli
  • Insert Size (bp)
    1257
  • Mutation
    mTurquoise2 inserted between Phe148 and Ser149
  • GenBank ID
    NC_000913.3
  • Entrez Gene
    ssb (a.k.a. b4059, ECK4051, exrB, lexC)
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • mTurquoise2

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAA TAC GAC TCA CTA TAG GG
  • 3′ sequencing primer GCT AGT TAT TGC TCA GCG G
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKD077 was a gift from James Keck (Addgene plasmid # 136473 ; http://n2t.net/addgene:136473 ; RRID:Addgene_136473)

  • For your References section:

    Development of a single-stranded DNA-binding protein fluorescent fusion toolbox. Dubiel K, Henry C, Spenkelink LM, Kozlov AG, Wood EA, Jergic S, Dixon NE, van Oijen AM, Cox MM, Lohman TM, Sandler SJ, Keck JL. Nucleic Acids Res. 2020 Jun 19;48(11):6053-6067. doi: 10.1093/nar/gkaa320. 10.1093/nar/gkaa320 PubMed 32374866
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