XLone-PE2
(Plasmid
#136463)
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PurposeExpresses prime editing 2 (PE2) enzyme upon doxycycline treatment
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 136463 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneXLone
- Total vector size (bp) 11961
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePE2
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Alt nameprime editing 2
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SpeciesSynthetic
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Insert Size (bp)6354
- Promoter TRE3GS
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer M13 FWD
- 3′ sequencing primer M13 REV (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byPE2 was from pCMV-PE2 (Plasmid #132775)
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XLone-PE2 was a gift from Xiaojun Lian (Addgene plasmid # 136463 ; http://n2t.net/addgene:136463 ; RRID:Addgene_136463)