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Addgene

XLone-PE2
(Plasmid #136463)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136463 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    XLone
  • Total vector size (bp) 11961
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PE2
  • Alt name
    prime editing 2
  • Species
    Synthetic
  • Insert Size (bp)
    6354
  • Promoter TRE3GS

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer M13 FWD
  • 3′ sequencing primer M13 REV
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    PE2 was from pCMV-PE2 (Plasmid #132775)
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XLone-PE2 was a gift from Xiaojun Lian (Addgene plasmid # 136463 ; http://n2t.net/addgene:136463 ; RRID:Addgene_136463)