pGEX2T E7 del 79-83
(Plasmid
#13646)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13646 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX2T
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 4900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHPV 16 E7 del 79-83
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Alt nameHPV 16 E7
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SpeciesH. sapiens (human)
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Insert Size (bp)300
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Mutationdel aa 79-83
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GenBank IDAY089955
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Entrez GeneE7 (a.k.a. HpV16gp2)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer Amersham pGEX 5'
- 3′ sequencing primer Amersham pGEX 3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX2T E7 del 79-83 was a gift from Karl Munger (Addgene plasmid # 13646 ; http://n2t.net/addgene:13646 ; RRID:Addgene_13646) -
For your References section:
The human papillomavirus E7 oncoprotein and the cellular transcription factor E2F bind to separate sites on the retinoblastoma tumor suppressor protein. Wu EW, Clemens KE, Heck DV, Munger K. J Virol. 1993 Apr . 67(4):2402-7. PubMed 8445736
