Skip to main content
Addgene

pCEP4-myc-PDGFRbeta
(Plasmid #136455)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136455 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10381
  • Total vector size (bp) 13729
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PDGFRbeta
  • Alt name
    PDGFRB
  • Alt name
    platelet-derived growth factor receptor beta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3348
  • GenBank ID
    NP_002600.1
  • Entrez Gene
    PDGFRB (a.k.a. CD140B, IBGC4, IMF1, JTK12, KOGS, PDGFR, PDGFR-1, PDGFR1, PENTT)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Signal peptide from influenza HA (N terminal on insert)
    • Extracellular c-myc epitope tag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-fwd
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4-myc-PDGFRbeta was a gift from Erik Procko (Addgene plasmid # 136455 ; http://n2t.net/addgene:136455 ; RRID:Addgene_136455)

  • For your References section:

    Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology. Park J, Gill KS, Aghajani AA, Heredia JD, Choi H, Oberstein A, Procko E. PLoS Pathog. 2020 Jun 19;16(6):e1008647. doi: 10.1371/journal.ppat.1008647. eCollection 2020 Jun. 10.1371/journal.ppat.1008647 PubMed 32559251
Related items:
Commonly requested with: