pcDNA3-HCMVgO-sfGFP (Merlin)
(Plasmid #136452)

• Purpose: Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) fused to superfolder GFP
• Depositing Lab: Erik Procko
• Publication: Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647. doi: 10.1371/journal.ppat.1008647. eCollection 2020 Jun.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5332
• Total vector size (bp): 7474
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: glycoprotein O
• Alt name: gO
• Alt name: UL74
• Species: Human betaherpesvirus 5
• Insert Size (bp): 2142
• Mutation: Codon-optimized for human cell expression.
• GenBank ID: AJY56739.1
• Entrez Gene: UL74 (a.k.a. HHV5wtgp068)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Signal peptide from human serum albumin (MKWVTFISLLFLFSSAYS) (N terminal on backbone)
  • Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI site) (C terminal on insert)
  • Superfolder GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-fwd
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry