pcDNA3-HCMVgH-8his (TB40/E)
(Plasmid #136445)

• Purpose: Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tag
• Depositing Lab: Erik Procko
• Publication: Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647. doi: 10.1371/journal.ppat.1008647. eCollection 2020 Jun.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5332
• Total vector size (bp): 7546
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: glycoprotein H
• Alt name: gH
• Alt name: UL75
• Species: Human betaherpesvirus 5
• Insert Size (bp): 2211
• Mutation: Extracellular domain (a.a. 1-717). Codon-optimized for human cell expression.
• GenBank ID: ABV71597.1
• Entrez Gene: UL75 (a.k.a. HHV5wtgp069)
• Promoter: CMV
• Tag / Fusion Protein:
  • Short GGSG linker and 8his tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-fwd
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry