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pcDNA3-sPDGFRalpha-Fc-V242K
(Plasmid #136440)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136440 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5332
  • Total vector size (bp) 7621
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PDGFRalpha
  • Alt name
    PDGFRA
  • Alt name
    platelet-derived growth factor receptor alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2289
  • Mutation
    Extracellular domain of PDGFR-alpha (a.a. 1-524) with V242K mutation. Binds HCMV trimer, but no longer binds PDGFs.
  • GenBank ID
    NP_006197.1
  • Entrez Gene
    PDGFRA (a.k.a. CD140A, PDGFR-2, PDGFR2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Linker GGGS (C terminal on insert)
    • Fc region of IgG1 (a.a. Asp-221 to Lys-447) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-fwd
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-sPDGFRalpha-Fc-V242K was a gift from Erik Procko (Addgene plasmid # 136440 ; http://n2t.net/addgene:136440 ; RRID:Addgene_136440)

  • For your References section:

    Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology. Park J, Gill KS, Aghajani AA, Heredia JD, Choi H, Oberstein A, Procko E. PLoS Pathog. 2020 Jun 19;16(6):e1008647. doi: 10.1371/journal.ppat.1008647. eCollection 2020 Jun. 10.1371/journal.ppat.1008647 PubMed 32559251
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