Skip to main content
Addgene

pcDNA3.1_mutNSDHL
(Plasmid #136428)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136428 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1_V5-HisA
  • Total vector size (bp) 6604
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human NSDHL with mutations in exon 4 and exon 6
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1131
  • Mutation
    C>T = A105V; G>C = A182P; G>A = R199H; G>A = G205S
  • Entrez Gene
    NSDHL (a.k.a. H105E3, SDR31E1, XAP104)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5-His tag (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1_mutNSDHL was a gift from Antonio Amelio (Addgene plasmid # 136428 ; http://n2t.net/addgene:136428 ; RRID:Addgene_136428)

  • For your References section:

    Identification of NSDHL mutations associated with CHILD syndrome in oral verruciform xanthoma. Getz GI, Parag-Sharma K, Reside J, Padilla RJ, Amelio AL. Oral Surg Oral Med Oral Pathol Oral Radiol. 2019 Jul;128(1):60-69. doi: 10.1016/j.oooo.2019.02.015. Epub 2019 Feb 23. 10.1016/j.oooo.2019.02.015 PubMed 31078502
Related items: