pJW31
(Plasmid #136423)

• Purpose: Contains genes of Type I-C CRISPR-Cas system for inducible expression and chromosomal integration at attTn7
• Depositing Lab: Joseph Bondy-Denomy
• Publication: Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s41592-020-00980-w. doi: 10.1038/s41592-020-00980-w.

Backbone

• Vector backbone: pUC18-mini-Tn7T-LAC
• Backbone manufacturer: Kyoung_Hee Choi, Herbert P. Schweizer
• Backbone size w/o insert (bp): 5815
• Total vector size (bp): 11380
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: also has Gentamicin resistance
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Cas5
• Species: P. aeruginosa
• Insert Size (bp): 654

Gene/Insert 2

• Gene/Insert name: Cas8
• Species: P. aeruginosa
• Insert Size (bp): 1764

Gene/Insert 3

• Gene/Insert name: Cas7
• Species: P. aeruginosa
• Insert Size (bp): 870

Gene/Insert 4

• Gene/Insert name: Cas3
• Species: P. aeruginosa
• Insert Size (bp): 2235

Gene/Insert 5

• Gene/Insert name: Cas3
• Species: P. aeruginosa
• Insert Size (bp): 2235

Resource Information

• Supplemental Documents:
  • pJW31.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry