Skip to main content
Addgene

pAEM7
(Plasmid #136422)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136422 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pHIS8
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 8000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    salL
  • Species
    Salinispora tropica CNB440
  • Insert Size (bp)
    852
  • Promoter T7
  • Tag / Fusion Protein
    • His8 tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAEM7 was a gift from Bradley Moore (Addgene plasmid # 136422 ; http://n2t.net/addgene:136422 ; RRID:Addgene_136422)

  • For your References section:

    Discovery and characterization of a marine bacterial SAM-dependent chlorinase. Eustaquio AS, Pojer F, Noel JP, Moore BS. Nat Chem Biol. 2008 Jan;4(1):69-74. doi: 10.1038/nchembio.2007.56. Epub 2007 Dec 2. 10.1038/nchembio.2007.56 PubMed 18059261
Related items: