pKLV-gRNA-CTBP2-MGMT_1
(Plasmid #136419)

• Purpose: Lentiviral expression of gRNAs targeting intron 1 of human CTBP2 and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.
• Depositing Lab: Massimo Squatrito
• Publication: Squatrito Lab Plasmids (unpublished)

Backbone

• Vector backbone: pKLV-U6gRNA-PGKpuro2ABFP
• Backbone manufacturer: Addgene #50946
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: U6_sgRNA(CTBP2)_U6_sgRNA(MGMT)
• gRNA/shRNA sequence: MGMT: TTTAACCAACGCAACACCGA; CTBP2: CGCGGGGGGAATTGGGAACC
• Species: H. sapiens (human)
• Promoter: U6

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pKLV-gRNA-CTBP2-MGMT_1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry