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Addgene

pKLV-gRNA-BTRC-MGMT_2
(Plasmid #136416)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136416 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKLV-U6gRNA-PGKpuro2ABFP
  • Backbone manufacturer
    Addgene #50946
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    U6_sgRNA(BTRC)_U6_sgRNA(MGMT)
  • gRNA/shRNA sequence
    MGMT: TTTAACCAACGCAACACCGA; BTRC: ACATAGTACAGCTTGACTGT
  • Species
    H. sapiens (human)
  • GenBank ID
  • Promoter U6

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKLV-gRNA-BTRC-MGMT_2 was a gift from Massimo Squatrito (Addgene plasmid # 136416 ; http://n2t.net/addgene:136416 ; RRID:Addgene_136416)
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