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pDONR-U6gRNA-PGKpuro2APDGFB
(Plasmid #136409)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136409 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR-U6gRNA-PGKpuro2ABFP
  • Modifications to backbone
    BbsI restriction site in the PDGFB sequence (Glu173Asp174) was removed by site directed mutagenesis. HA tag on vector.
  • Vector type
    CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Mutation
    BbsI restriction site in the PDGFB sequence (Glu173Asp174) was removed by site directed mutagenesis

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR-U6gRNA-PGKpuro2APDGFB was a gift from Massimo Squatrito (Addgene plasmid # 136409 ; http://n2t.net/addgene:136409 ; RRID:Addgene_136409)

  • For your References section:

    Somatic genome editing with the RCAS-TVA-CRISPR-Cas9 system for precision tumor modeling. Oldrini B, Curiel-Garcia A, Marques C, Matia V, Uluckan O, Grana-Castro O, Torres-Ruiz R, Rodriguez-Perales S, Huse JT, Squatrito M. Nat Commun. 2018 Apr 13;9(1):1466. doi: 10.1038/s41467-018-03731-w. 10.1038/s41467-018-03731-w PubMed 29654229
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