pDONR-U6gRNA-PGKpuro2ABFP
(Plasmid
#136405)
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Purpose(Empty Backbone) Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 136405 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR221
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Modifications to backboneBbsI restriction site at position 437 was removed by site directed mutagenesis.
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Vector typeCRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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MutationBbsI restriction site at position 437 was removed by site directed mutagenesis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR-U6gRNA-PGKpuro2ABFP was a gift from Massimo Squatrito (Addgene plasmid # 136405 ; http://n2t.net/addgene:136405 ; RRID:Addgene_136405) -
For your References section:
Somatic genome editing with the RCAS-TVA-CRISPR-Cas9 system for precision tumor modeling. Oldrini B, Curiel-Garcia A, Marques C, Matia V, Uluckan O, Grana-Castro O, Torres-Ruiz R, Rodriguez-Perales S, Huse JT, Squatrito M. Nat Commun. 2018 Apr 13;9(1):1466. doi: 10.1038/s41467-018-03731-w. 10.1038/s41467-018-03731-w PubMed 29654229
