pPS2169
(Plasmid
#1364)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1364 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPS1530
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameRPL11B
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneRPL11B
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
CEN vector with ORF of GFP followed by the 3'UTR of NUF2.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPS2169 was a gift from Pamela Silver (Addgene plasmid # 1364 ; http://n2t.net/addgene:1364 ; RRID:Addgene_1364) -
For your References section:
Factors affecting nuclear export of the 60S ribosomal subunit in vivo. Stage-Zimmermann T, Schmidt U, Silver PA. Mol Biol Cell 2000 Nov;11(11):3777-89. 10.1091/mbc.11.11.3777 PubMed 11071906