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pJ414 - NERD-C
(Plasmid #136389)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136389 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ414
  • Backbone manufacturer
    ATUM (formally DNA 2.0)
  • Backbone size w/o insert (bp) 6200
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NERD-C
  • Alt name
    Streptococcal protein G, domain B1 mutant
  • Species
    Streptococcal bacteria
  • Insert Size (bp)
    168
  • Mutation
    Mutation from GB1 WT: Y3F-L7I-F30L-V39I
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • 6x-His tag (N-term of insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer NdeI restriction site
  • 3′ sequencing primer XhoI restriction site
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ATUM (Formally DNA 2.0)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJ414 - NERD-C was a gift from Roberto Chica (Addgene plasmid # 136389 ; http://n2t.net/addgene:136389 ; RRID:Addgene_136389)

  • For your References section:

    Rational design of proteins that exchange on functional timescales. Davey JA, Damry AM, Goto NK, Chica RA. Nat Chem Biol. 2017 Dec;13(12):1280-1285. doi: 10.1038/nchembio.2503. Epub 2017 Oct 23. 10.1038/nchembio.2503 PubMed 29058725
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