Skip to main content
Addgene

pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR
(Plasmid #136382)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136382 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pYPQ167
  • Vector type
    Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AaCas12b (D570A)-TV-T2A-MCP-VPR
  • Species
    Alicyclobacillus acidiphilus
  • Mutation
    D570A

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TV activation system is from: Li, Z., Zhang, D., Xiong, X. et al. A potent Cas9-derived gene activator for plant and mammalian cells. Nature Plants 3, 930–936 (2017) doi:10.1038/s41477-017-0046-3; VPR activation system is from: Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, E PRI, et al. Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015;12(4):326–8. 10.1038/nmeth.3312

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR was a gift from Yiping Qi (Addgene plasmid # 136382 ; http://n2t.net/addgene:136382 ; RRID:Addgene_136382)

  • For your References section:

    CRISPR-Cas12b enables efficient plant genome engineering. Ming M, Ren Q, Pan C, He Y, Zhang Y, Liu S, Zhong Z, Wang J, Malzahn AA, Wu J, Zheng X, Zhang Y, Qi Y. Nat Plants. 2020 Mar;6(3):202-208. doi: 10.1038/s41477-020-0614-6. Epub 2020 Mar 9. 10.1038/s41477-020-0614-6 PubMed 32170285
Related items:
Commonly requested with: