pYPQ141-ZmUbi-RZ-Aac.3
(Plasmid
#136377)
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Purpose(Empty Backbone) Gateway entry clone for AacCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold with MS2 at the third stem loop is used
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 136377 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYPQ141-ZmUbi-RZ-Lb
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Vector typePlant Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYPQ141-ZmUbi-RZ-Aac.3 was a gift from Yiping Qi (Addgene plasmid # 136377 ; http://n2t.net/addgene:136377 ; RRID:Addgene_136377) -
For your References section:
CRISPR-Cas12b enables efficient plant genome engineering. Ming M, Ren Q, Pan C, He Y, Zhang Y, Liu S, Zhong Z, Wang J, Malzahn AA, Wu J, Zheng X, Zhang Y, Qi Y. Nat Plants. 2020 Mar;6(3):202-208. doi: 10.1038/s41477-020-0614-6. Epub 2020 Mar 9. 10.1038/s41477-020-0614-6 PubMed 32170285