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Addgene

pMXs_FLAG-MFT
(Plasmid #136371)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136371 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMXs
  • Backbone size w/o insert (bp) 5600
  • Total vector size (bp) 6600
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MFT
  • Alt name
    SLC25A32
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1050
  • Mutation
    codon-optimized
  • Entrez Gene
    SLC25A32 (a.k.a. MFT, MFTC, RREI)
  • Tag / Fusion Protein
    • 3XFlag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer GTGGACCATCCTCTAG
  • 3′ sequencing primer GACGGCAATATGGTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs_FLAG-MFT was a gift from David Sabatini (Addgene plasmid # 136371 ; http://n2t.net/addgene:136371 ; RRID:Addgene_136371)

  • For your References section:

    MCART1/SLC25A51 is required for mitochondrial NAD transport. Kory N, Uit de Bos J, van der Rijt S, Jankovic N, Gura M, Arp N, Pena IA, Prakash G, Chan SH, Kunchok T, Lewis CA, Sabatini DM. Sci Adv. 2020 Oct 21;6(43):eabe5310. doi: 10.1126/sciadv.abe5310. Print 2020 Oct. 10.1126/sciadv.abe5310 PubMed 33087354
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