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Addgene

pGEX2T AtFC1
(Plasmid #136363)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136363 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-2T
  • Backbone manufacturer
    GE Healthcare Life Sciences
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Full-length cDNA of Arabidopsis thaliana ferrochelatase1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1401
  • Entrez Gene
    FC1 (a.k.a. AT5G26030, ATFC-I, FC-I, T1N24.17, T1N24_17, ferrochelatase 1)
  • Promoter Lac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX2T AtFC1 was a gift from Tatsuru Masuda (Addgene plasmid # 136363 ; http://n2t.net/addgene:136363 ; RRID:Addgene_136363)

  • For your References section:

    The retrograde signaling protein GUN1 regulates tetrapyrrole biosynthesis. Shimizu T, Kacprzak SM, Mochizuki N, Nagatani A, Watanabe S, Shimada T, Tanaka K, Hayashi Y, Arai M, Leister D, Okamoto H, Terry MJ, Masuda T. Proc Natl Acad Sci U S A. 2019 Dec 3;116(49):24900-24906. doi: 10.1073/pnas.1911251116. Epub 2019 Nov 15. 10.1073/pnas.1911251116 PubMed 31732672
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