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Addgene

pET48 AtGUN1-PS
(Plasmid #136358)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136358 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET48(+)
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use Rosseta gami2 (DE3) for protein expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 918
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    2058
  • Entrez Gene
    GUN1 (a.k.a. AT2G31400, T28P16.11, T28P16_11, genomes uncoupled 1)
  • Promoter T7
  • Tag / Fusion Protein
    • TRX-His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET48 AtGUN1-PS was a gift from Tatsuru Masuda (Addgene plasmid # 136358 ; http://n2t.net/addgene:136358 ; RRID:Addgene_136358)

  • For your References section:

    The retrograde signaling protein GUN1 regulates tetrapyrrole biosynthesis. Shimizu T, Kacprzak SM, Mochizuki N, Nagatani A, Watanabe S, Shimada T, Tanaka K, Hayashi Y, Arai M, Leister D, Okamoto H, Terry MJ, Masuda T. Proc Natl Acad Sci U S A. 2019 Dec 3;116(49):24900-24906. doi: 10.1073/pnas.1911251116. Epub 2019 Nov 15. 10.1073/pnas.1911251116 PubMed 31732672
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