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pLENTI_Hu_ECT2(T327D)
(Plasmid #136332)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136332 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLENTI_CMV_Puro_DEST
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ECT2
  • Species
    H. sapiens (human)
  • Mutation
    T327D
  • Entrez Gene
    ECT2 (a.k.a. ARHGEF31)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLENTI_Hu_ECT2(T327D) was a gift from Guillermo de Cárcer & Marcos Malumbres (Addgene plasmid # 136332 ; http://n2t.net/addgene:136332 ; RRID:Addgene_136332)

  • For your References section:

    Plk1 regulates contraction of postmitotic smooth muscle cells and is required for vascular homeostasis. de Carcer G, Wachowicz P, Martinez-Martinez S, Oller J, Mendez-Barbero N, Escobar B, Gonzalez-Loyola A, Takaki T, El Bakkali A, Camara JA, Jimenez-Borreguero LJ, Bustelo XR, Canamero M, Mulero F, de Los Angeles Sevilla M, Montero MJ, Redondo JM, Malumbres M. Nat Med. 2017 Aug;23(8):964-974. doi: 10.1038/nm.4364. Epub 2017 Jul 10. 10.1038/nm.4364 PubMed 28692064
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