EGFP-Mm_Plk5(300-595) PBD domain
(Plasmid
#136316)
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PurposeMammalian expression of mouse Plk5-D300 (PBD domain 300-595) with N-terminal EGFP
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 136316 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1-EGFP
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePLK5
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SpeciesM. musculus (mouse)
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Mutationfragment 300-595
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Entrez GenePlk5 (a.k.a. 6330514A18Rik)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP-Mm_Plk5(300-595) PBD domain was a gift from Guillermo de Cárcer & Marcos Malumbres (Addgene plasmid # 136316 ; http://n2t.net/addgene:136316 ; RRID:Addgene_136316) -
For your References section:
Plk5, a polo box domain-only protein with specific roles in neuron differentiation and glioblastoma suppression. de Carcer G, Escobar B, Higuero AM, Garcia L, Anson A, Perez G, Mollejo M, Manning G, Melendez B, Abad-Rodriguez J, Malumbres M. Mol Cell Biol. 2011 Mar;31(6):1225-39. doi: 10.1128/MCB.00607-10. Epub 2011 Jan 18. 10.1128/MCB.00607-10 PubMed 21245385