pAEF5
(Plasmid
#136305)
-
Purpose(Empty Backbone) Plasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin Fleiss
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 136305 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepGangZhao110
-
Backbone manufacturerBruce Futcher laboratory at Stony Brooks University
- Backbone size (bp) 10864
-
Modifications to backboneleu2 gene on the pGangZhao110 is replaced by hph gene
-
Vector typeCRISPR
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Resource Information
-
Supplemental Documents
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAEF5 was a gift from Gilles Fischer (Addgene plasmid # 136305 ; http://n2t.net/addgene:136305 ; RRID:Addgene_136305) -
For your References section:
A Versatile Protocol to Generate Translocations in Yeast Genomes Using CRISPR/Cas9. Agier N, Fleiss A, Delmas S, Fischer G. Methods Mol Biol. 2021;2196:181-198. doi: 10.1007/978-1-0716-0868-5_14. 10.1007/978-1-0716-0868-5_14 PubMed 32889721
Map uploaded by the depositor.
