Skip to main content
Addgene

pAEF6
(Plasmid #136303)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136303 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGangZhao110
  • Backbone manufacturer
    Bruce Futcher laboratory at Stony Brooks University
  • Backbone size w/o insert (bp) 10864
  • Modifications to backbone
    leu2 gene on the pGangZhao110 is replaced by hph gene
  • Vector type
    CRISPR ; template for PCR to insert double gRNA coding sequence in pAEF5
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    double cassette coding for gRNA
  • Species
    Synthetic
  • Insert Size (bp)
    410

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAEF6 was a gift from Gilles Fischer (Addgene plasmid # 136303 ; http://n2t.net/addgene:136303 ; RRID:Addgene_136303)

  • For your References section:

    A Versatile Protocol to Generate Translocations in Yeast Genomes Using CRISPR/Cas9. Agier N, Fleiss A, Delmas S, Fischer G. Methods Mol Biol. 2021;2196:181-198. doi: 10.1007/978-1-0716-0868-5_14. 10.1007/978-1-0716-0868-5_14 PubMed 32889721
Related items:
Commonly requested with: