ABE8.17-m
(Plasmid #136298)

• Purpose: expresses ABE8.17-m in mammalian cells
• Depositing Lab: Nicole Gaudelli
• Publication: Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6.

Backbone

• Vector backbone: pBR322, beta-lactamase maintenance, CMV promoter
• Backbone manufacturer: none
• Backbone size w/o insert (bp): 3400
• Total vector size (bp): 8166
• Modifications to backbone: none
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ABE8.17-m
• Insert Size (bp): 4800
• Mutation: D10A in S. pyogenes Cas9, TadA mutations described in manuscript
• Tag / Fusion Protein:
  • C-terminal BPNLS (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • ABE8.17-m.gb
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.03.13.990630v1 for bioRxiv preprint.