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Addgene

GST-RH▲-MNase
(Plasmid #136292)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136292 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6P-1
  • Backbone size w/o insert (bp) 4966
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    TOP10/BL21
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RNase HI-D10R-E48R and MNase
  • Species
    Escherichia coli and Staphylococcus aureus
  • Insert Size (bp)
    465

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-RH▲-MNase was a gift from Kavitha Sarma (Addgene plasmid # 136292 ; http://n2t.net/addgene:136292 ; RRID:Addgene_136292)

  • For your References section:

    Mapping Native R-Loops Genome-wide Using a Targeted Nuclease Approach. Yan Q, Shields EJ, Bonasio R, Sarma K. Cell Rep. 2019 Oct 29;29(5):1369-1380.e5. doi: 10.1016/j.celrep.2019.09.052. 10.1016/j.celrep.2019.09.052 PubMed 31665646

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