pQE30 NFAT5 DBD
(Plasmid
#13629)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13629 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepQE30
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Backbone manufacturerQiagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNFAT5
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SpeciesH. sapiens (human)
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Insert Size (bp)900
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MutationDNA binding domain: aa 175 to 471
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Entrez GeneNFAT5 (a.k.a. NF-AT5, NFATL1, NFATZ, OREBP, TONEBP)
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Tag
/ Fusion Protein
- 6x His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQE30 NFAT5 DBD was a gift from Anjana Rao (Addgene plasmid # 13629 ; http://n2t.net/addgene:13629 ; RRID:Addgene_13629) -
For your References section:
NFAT5, a constitutively nuclear NFAT protein that does not cooperate with Fos and Jun. Lopez-Rodriguez C, Aramburu J, Rakeman AS, Rao A. Proc Natl Acad Sci U S A. 1999 Jun 22. 96(13):7214-9. 10.1073/pnas.96.13.7214 PubMed 10377394