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Addgene

pLB052 - deAct - SpVB
(Plasmid #136286)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136286 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    D000
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 3345
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    deAct - SpvB
  • Alt name
    mono(ADP-ribosyl) transferase domain of the Salmonella enterica SpvB
  • Species
    Salmonella enterica
  • Insert Size (bp)
    648
  • GenBank ID
    39691373

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco31I (not destroyed)
  • 3′ cloning site Eco31I (not destroyed)
  • 5′ sequencing primer ATTTAGGTGACACTATAG
  • 3′ sequencing primer AATACGACTCACTATAGGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLB052 - deAct - SpVB was a gift from Alexis Maizel (Addgene plasmid # 136286 ; http://n2t.net/addgene:136286 ; RRID:Addgene_136286)

  • For your References section:

    Cytoskeleton Dynamics Are Necessary for Early Events of Lateral Root Initiation in Arabidopsis. Vilches Barro A, Stockle D, Thellmann M, Ruiz-Duarte P, Bald L, Louveaux M, von Born P, Denninger P, Goh T, Fukaki H, Vermeer JEM, Maizel A. Curr Biol. 2019 Aug 5;29(15):2443-2454.e5. doi: 10.1016/j.cub.2019.06.039. Epub 2019 Jul 18. 10.1016/j.cub.2019.06.039 PubMed 31327713
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