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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13627 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1 (EGFP removed)
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNFAT5
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SpeciesH. sapiens (human)
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Insert Size (bp)4400
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Entrez GeneNFAT5 (a.k.a. NF-AT5, NFATL1, NFATZ, OREBP, TONEBP)
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Tag
/ Fusion Protein
- 6xMyc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
BamHI-6xMyc-EcoRI-NFAT5. The EGFP gene is not present in this vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP NFAT5 (no EGFP) was a gift from Anjana Rao (Addgene plasmid # 13627 ; http://n2t.net/addgene:13627 ; RRID:Addgene_13627) -
For your References section:
NFAT5, a constitutively nuclear NFAT protein that does not cooperate with Fos and Jun. Lopez-Rodriguez C, Aramburu J, Rakeman AS, Rao A. Proc Natl Acad Sci U S A. 1999 Jun 22. 96(13):7214-9. 10.1073/pnas.96.13.7214 PubMed 10377394