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Addgene

pEGFP NFAT5 (no EGFP)
(Plasmid #13627)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13627 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-N1 (EGFP removed)
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NFAT5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4400
  • Entrez Gene
    NFAT5 (a.k.a. NF-AT5, NFATL1, NFATZ, OREBP, TONEBP)
  • Tag / Fusion Protein
    • 6xMyc (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

BamHI-6xMyc-EcoRI-NFAT5. The EGFP gene is not present in this vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP NFAT5 (no EGFP) was a gift from Anjana Rao (Addgene plasmid # 13627 ; http://n2t.net/addgene:13627 ; RRID:Addgene_13627)
  • For your References section:

    NFAT5, a constitutively nuclear NFAT protein that does not cooperate with Fos and Jun. Lopez-Rodriguez C, Aramburu J, Rakeman AS, Rao A. Proc Natl Acad Sci U S A. 1999 Jun 22. 96(13):7214-9. 10.1073/pnas.96.13.7214 PubMed 10377394