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Addgene

pcDNA4-Claspin-Flag
(Plasmid #136246)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136246 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Claspin
  • Species
    H. sapiens (human)
  • Mutation
    E263G, Q499R, I783T, D932G and S1062G- Please see depositor comments below
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor confirms mutations does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4-Claspin-Flag was a gift from Aziz Sancar (Addgene plasmid # 136246 ; http://n2t.net/addgene:136246 ; RRID:Addgene_136246)

  • For your References section:

    Human claspin is a ring-shaped DNA-binding protein with high affinity to branched DNA structures. Sar F, Lindsey-Boltz LA, Subramanian D, Croteau DL, Hutsell SQ, Griffith JD, Sancar A. J Biol Chem. 2004 Sep 17;279(38):39289-95. doi: 10.1074/jbc.M405793200. Epub 2004 Jun 28. 10.1074/jbc.M405793200 PubMed 15226314
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