pcDNA4-Claspin-Flag
(Plasmid
#136246)
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PurposeProtein expression of Claspin with a C terminal FLAG tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 136246 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA4
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameClaspin
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SpeciesH. sapiens (human)
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MutationE263G, Q499R, I783T, D932G and S1062G- Please see depositor comments below
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Depositor confirms mutations does not affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA4-Claspin-Flag was a gift from Aziz Sancar (Addgene plasmid # 136246 ; http://n2t.net/addgene:136246 ; RRID:Addgene_136246) -
For your References section:
Human claspin is a ring-shaped DNA-binding protein with high affinity to branched DNA structures. Sar F, Lindsey-Boltz LA, Subramanian D, Croteau DL, Hutsell SQ, Griffith JD, Sancar A. J Biol Chem. 2004 Sep 17;279(38):39289-95. doi: 10.1074/jbc.M405793200. Epub 2004 Jun 28. 10.1074/jbc.M405793200 PubMed 15226314
