pGex4T1-JET
(Plasmid
#136245)
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PurposeProtein expression of Jetlag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 136245 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX4T1
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Backbone manufacturerGE
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameJetlag
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SpeciesD. melanogaster (fly)
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGex4T1-JET was a gift from Aziz Sancar (Addgene plasmid # 136245 ; http://n2t.net/addgene:136245 ; RRID:Addgene_136245) -
For your References section:
Mechanism of photosignaling by Drosophila cryptochrome: role of the redox status of the flavin chromophore. Ozturk N, Selby CP, Zhong D, Sancar A. J Biol Chem. 2014 Feb 21;289(8):4634-42. doi: 10.1074/jbc.M113.542498. Epub 2013 Dec 30. 10.1074/jbc.M113.542498 PubMed 24379403
