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Addgene

pcDNA4-ZfCry4
(Plasmid #136242)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136242 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4/mycHis
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZfCry4
  • Species
    D. rerio (zebrafish)
  • Mutation
    D196H- Please see depositor comments below
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor confirms mutation D196H in zfCry4 does not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4-ZfCry4 was a gift from Aziz Sancar (Addgene plasmid # 136242 ; http://n2t.net/addgene:136242 ; RRID:Addgene_136242)

  • For your References section:

    Comparative photochemistry of animal type 1 and type 4 cryptochromes. Ozturk N, Selby CP, Song SH, Ye R, Tan C, Kao YT, Zhong D, Sancar A. Biochemistry. 2009 Sep 15;48(36):8585-93. doi: 10.1021/bi901043s. 10.1021/bi901043s PubMed 19663499
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