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Addgene

pFast-dCry-V5His
(Plasmid #136240)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136240 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DmCry
  • Species
    D. melanogaster (fly)
  • Tags / Fusion Proteins
    • V5 (C terminal on insert)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFast-dCry-V5His was a gift from Aziz Sancar (Addgene plasmid # 136240 ; http://n2t.net/addgene:136240 ; RRID:Addgene_136240)

  • For your References section:

    Comparative photochemistry of animal type 1 and type 4 cryptochromes. Ozturk N, Selby CP, Song SH, Ye R, Tan C, Kao YT, Zhong D, Sancar A. Biochemistry. 2009 Sep 15;48(36):8585-93. doi: 10.1021/bi901043s. 10.1021/bi901043s PubMed 19663499
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