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Addgene

pAC5.1-bgal-V5His
(Plasmid #136238)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136238 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAc5.1/V5-HisA
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Beta-galactosidase
  • Species
    E. coli
  • Tags / Fusion Proteins
    • V5 (C terminal on insert)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC5.1-bgal-V5His was a gift from Steven Reppert & Aziz Sancar (Addgene plasmid # 136238 ; http://n2t.net/addgene:136238 ; RRID:Addgene_136238)

  • For your References section:

    The two CRYs of the butterfly. Zhu H, Yuan Q, Briscoe AD, Froy O, Casselman A, Reppert SM. Curr Biol. 2005 Dec 6;15(23):R953-4. doi: 10.1016/j.cub.2005.11.030. 10.1016/j.cub.2005.11.030 PubMed 16332522
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