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pFl Strep-sumo-TEV-hAgo2 D597N
(Plasmid #136236)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136236 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFl
  • Backbone size w/o insert (bp) 6549
  • Total vector size (bp) 9129
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human Argonaute-2
  • Alt name
    hAgo2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2580
  • Mutation
    D597N
  • Entrez Gene
    AGO2 (a.k.a. CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10)
  • Promoter polyhedrin
  • Tag / Fusion Protein
    • Sterp Sumo (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer MCS2 FW
  • 3′ sequencing primer MCS2 RV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFl Strep-sumo-TEV-hAgo2 D597N was a gift from Leemor Joshua-Tor (Addgene plasmid # 136236 ; http://n2t.net/addgene:136236 ; RRID:Addgene_136236)

  • For your References section:

    siRNA carrying an (E)-vinylphosphonate moiety at the 5 end of the guide strand augments gene silencing by enhanced binding to human Argonaute-2. Elkayam E, Parmar R, Brown CR, Willoughby JL, Theile CS, Manoharan M, Joshua-Tor L. Nucleic Acids Res. 2017 Apr 7;45(6):3528-3536. doi: 10.1093/nar/gkw1171. 10.1093/nar/gkw1171 PubMed 27903888
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