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Addgene

pPS1890
(Plasmid #1362)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1362 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS304
  • Backbone size w/o insert (bp) 4271
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLS-ECFP
  • Alt name
    pRS304-NLS-ECFP
  • Alt name
    ECFP
  • Species
    NA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ECFP, NUF2 3' UTR, integrating vector with TRP1 marker for C-terminal ECFP fusions

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS1890 was a gift from Pamela Silver (Addgene plasmid # 1362 ; http://n2t.net/addgene:1362 ; RRID:Addgene_1362)

  • For your References section:

    Mapping interactions between nuclear transport factors in living cells reveals pathways through the nuclear pore complex. Damelin M, Silver PA. Mol Cell 2000 Jan;5(1):133-40. 10.1016/S1097-2765(00)80409-8 PubMed 10678175
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