pcDNA3 PARL-FLAG-CT S65A+T69A+S70A
(Plasmid #13616)

• Depositing Lab: Luca Pellegrini
• Publication: Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 103(49):18562-7.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Presenilin-associated rhomboid-like
• Alt name: PARL
• Species: H. sapiens (human)
• Insert Size (bp): 1164
• Mutation: changed Ser 65, Thr 69 and Ser 70 to Alanine
• GenBank ID: AF197937
• Entrez Gene: PARL (a.k.a. PRO2207, PSARL, PSARL1, PSENIP2, RHBDS1)
• Entrez Gene: PARL (a.k.a. PARL)
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: GTACGGTGGGAGGTCTATAT

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry