Skip to main content
Addgene

[pSK729] pRK5 HA-FLCN(R164A)
(Plasmid #136147)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136147 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLCN
  • Species
    H. sapiens (human)
  • Mutation
    R164A
  • Entrez Gene
    FLCN (a.k.a. BHD, DENND8B, FLCL)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    [pSK729] pRK5 HA-FLCN(R164A) was a gift from David Sabatini & Kuang Shen (Addgene plasmid # 136147 ; http://n2t.net/addgene:136147 ; RRID:Addgene_136147)

  • For your References section:

    Cryo-EM Structure of the Human FLCN-FNIP2-Rag-Ragulator Complex. Shen K, Rogala KB, Chou HT, Huang RK, Yu Z, Sabatini DM. Cell. 2019 Nov 27;179(6):1319-1329.e8. doi: 10.1016/j.cell.2019.10.036. Epub 2019 Nov 6. 10.1016/j.cell.2019.10.036 PubMed 31704029
Related items:
Commonly requested with: