[pSK24] pACYCDuet His-c7orf59|HBXIP
(Plasmid
#136143)
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Purposebacterial expression of c7orf59 and HBXIP
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 136143 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACYCDuet
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
[pSK24] pACYCDuet His-c7orf59|HBXIP was a gift from David Sabatini & Kuang Shen (Addgene plasmid # 136143 ; http://n2t.net/addgene:136143 ; RRID:Addgene_136143) -
For your References section:
Ragulator and SLC38A9 activate the Rag GTPases through noncanonical GEF mechanisms. Shen K, Sabatini DM. Proc Natl Acad Sci U S A. 2018 Sep 18;115(38):9545-9550. doi: 10.1073/pnas.1811727115. Epub 2018 Sep 4. 10.1073/pnas.1811727115 PubMed 30181260