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Addgene

[pSK581] pRK5 HA-SLC38A9N
(Plasmid #136141)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136141 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SLC38A9 N term fragment (1-119)
  • Species
    H. sapiens (human)
  • GenBank ID
  • Entrez Gene
    SLC38A9 (a.k.a. URLC11)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    [pSK581] pRK5 HA-SLC38A9N was a gift from David Sabatini & Kuang Shen (Addgene plasmid # 136141 ; http://n2t.net/addgene:136141 ; RRID:Addgene_136141)

  • For your References section:

    Ragulator and SLC38A9 activate the Rag GTPases through noncanonical GEF mechanisms. Shen K, Sabatini DM. Proc Natl Acad Sci U S A. 2018 Sep 18;115(38):9545-9550. doi: 10.1073/pnas.1811727115. Epub 2018 Sep 4. 10.1073/pnas.1811727115 PubMed 30181260
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