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Addgene

integrin alpha9 DM3 pWZL Blast2
(Plasmid #13613)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13613 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWZL Blast2
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    integrin alpha 9 DM3
  • Alt name
    ITGA9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3100
  • Mutation
    C-terminal deletion mutant missing the last 22 amino acids in the cytoplasmic domain.
  • GenBank ID
    NM_002207
  • Entrez Gene
    ITGA9 (a.k.a. ALPHA-RLC, ITGA4L, RLC)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII/SnaBI (destroyed during cloning)
  • 3′ cloning site XbaI/SnaBI (destroyed during cloning)
  • 5′ sequencing primer pBABE 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for additional restriction enzyme sites.

Please see Young et al. (2001), MBC 12:3214 for information regarding which amino acids are removed in this deletion construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    integrin alpha9 DM3 pWZL Blast2 was a gift from Dean Sheppard (Addgene plasmid # 13613 ; http://n2t.net/addgene:13613 ; RRID:Addgene_13613)
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