pGEX:PGRMC1
(Plasmid #136062)

• Purpose: Bacterial expression of pGEX:PGRMC1 to perform a GST-pulldown assay and identify new proteins that interact with GST-PGRMC1 in the human endometrial stroll cells.
• Depositing Lab: Francisco Domínguez Hernández
• Publication: Salsano et al Fertil Steril. 2020 May;113(5):1050-1066.e7. doi: 10.1016/j.fertnstert.2020.01.008.

Backbone

• Vector backbone: pGEX6P-1
• Backbone size w/o insert (bp): 4984
• Total vector size (bp): 5569
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Progesterone receptor membrane component 1
• Alt name: PGRMC1
• Species: H. sapiens (human)
• Insert Size (bp): 585
• GenBank ID: 10857
• Entrez Gene: PGRMC1 (a.k.a. Dap1, HPR6.6, IZA, MPR)
• Tag / Fusion Protein:
  • GST-PGRMC1 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: 5’GGATCCatggctgccgaggatgtggtg3’
• 3′ sequencing primer: 5’GAATTCatcatttttccgggcact3’

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry